Anti-cancer effects of Kochia scoparia fruit in human breast cancer
cells.
Han HY, Kim H, Son YH, Lee G, Jeong SH, Ryu MH.
Abstract
BACKGROUND:
The fruit of Kochia scoparia Scharder is widely used as a medicinal ingredient
for the treatment of dysuria and skin diseases in China, Japan and Korea. Especially,
K. scoparia had been used for breast masses and chest and flank pain.
OBJECTIVE:
To investigate the anti-cancer effect of K. scoparia on breast cancer.
MATERIALS AND METHODS:
We investigated the anti-cancer effects of K. scoparia, methanol extract (MEKS)
in vitro. We examined the effects of MEKS on the proliferation rate, cell cycle
arrest, reactive oxygen species (ROS) generation and activation of apoptosis-associated
proteins in MDA-MB-231, human breast cancer cells.
RESULTS:
MTT assay results demonstrated that MEKS decreased the proliferation rates of
MDA-MB-231 cells in a dose-dependent manner with an IC50 value of 36.2 μg/ml.
MEKS at 25 μg/ml significantly increased the sub-G1 DNA contents of MDA-MB-231
cells to 44.7%, versus untreated cells. In addition, MEKS induced apoptosis
by increasing the levels of apoptosis-associated proteins such as cleaved caspase
3, cleaved caspase 8, cleaved caspase 9 and cleaved Poly (ADP-ribose) polymerase
(PARP).
CONCLUSION:
These results suggest that MEKS inhibits cell proliferation and induces apoptosis
in breast cancer cells and that MEKS may have potential chemotherapeutic value
for the treatment of human breast cancer. Pharmacogn Mag. 2014 Aug;10(Suppl
3):S661-7. doi: 10.4103/0973-1296.139812. ncbi.nlm.nih.gov
Acaricidal activities of extracts of Kochia scoparia against Tetranychus
urticae, Tetranychus cinnabarinus, and Tetranychus viennensis (Acari: Tetranychidae).
Shi GL, Zhao LL, Liu SQ, Cao H, Clarke SR, Sun JH.
Abstract
Extracts of an annual herbaceous plant, Kochia scoparia (L.) Schrad (Macrophomina),
were bioassayed to determine their acaricidal activities against Tetranychus
urticae Koch, Tetranychus cinnabarinus (Boisduval), and Tetranychus viennensis
Zacher (Acari: Tetranychidae) in the laboratory. Extracts had both contact and
systemic toxicity to these mites. Three solvents were tested for preparing crude
extracts: petroleum ether, chloroform, and methanol. Methanol was the most effective
solvent, extracting 3.11-4.53% of the acaricide. Petroleum ether was the least
effective solvent, extracting 1.25-1.54%. However, extracts with chloroform
resulted in the highest mite mortality (78.86%), and ultrasound-assisted extraction
required the least time (10 min). Concentrated extracts were prepared using
chloroform, methyl acetate, or distilled water as a solvent. Mite mortalities
from the concentrated extracts by methyl acetate or distilled water were significantly
lower than those by chloroform. The mean lethal concentrations (LC50) of the
extracts by chloroform, methyl acetate, and distilled water to the mites were
0.71 +/- 0.06, 2.08 +/- 0.16 and 8.75 +/- 0.062 mg/ml, respectively. After liquid
chromatography and thin layer chromatography, the concentrated extracts by chloroform
were separated into seven groups of isolated fractions and tested for acaricidal
activity. J Econ Entomol. 2006 Jun;99(3):858-63 ncbi.nlm.nih.gov