Lasiosphaera fenzlii, L. nipponina and Calvatia gigantea and C. lilacina   马 勃  Mǎ bó- "horse thriving"    Puff-ball      
PART USED: Fruiting body of puffball- harvested mid Summer to beginning Autumn.
    FLAVOR: Pungent, acrid      CHANNEL: Lung
FUNCTIONS
GROUP: Clearing Internal Heat- Neutralizing Toxins
1. Clears Lung,[2,3] relieves Fire toxicity.[2,3]
2. Clear Heat.[1,2] Benefit throat.[1,2]
3. Arrest bleeding,[1,2,3] used topically.[3]
INDICATIONS
1. Fire toxin affecting the throat;[3] Sore throat, pain swelling, hoarseness and loss of voice.[3]
2. Cough due to Lung heat; cough, hoarseness.[2,3] Upper respiratory tract infections.[2] Bleeding in oral cavity or lips.[3] Bleeding gums associated with frostbite.[3]
3. External applications to stop local bleeding: nosebleed and bleeding due to external injuries. Cracked skin. Use powder of herb and rub onto skin.[2]
CONTRAINDICATIONS: Loss of voice due to Wind Cold.[3]
COMBINATIONS
PREPARATIONS: Dried seeds body 1-3 g.[1] 1-4.5 g[3] Wrap in cheesecloth while cooking. Use topically as a powder to stop bleeding or for sore, swollen throat.[3] Good quality is large, full, and elastic and has a thin skin.
References
Inner Path can not take any responsibility for any adverse effects from the use of plants. Always seek advice from a professional before using a plant medicinally.
Constituents
Research

WLIP derived from Lasiosphaera fenzlii Reich exhibits anti-tumor activity and induces cell cycle arrest through PPAR-γ associated pathways.
Meng J, Fan Y, Su M, Chen C, Ren T, Wang J, Zhao Q.
Abstract
White-line-inducing principle (WLIP), a lipodepsipeptide isolated from Lasiosphaera fenzlii Reich., which is one of the puffballs, for the first time, has been reported to exhibit anti-microbial activity. However, there are no reports regarding the anti-tumor effects of WLIP. In this study, we reported the anti-cancer effects of WLIP on K562 cells, and its potential effect on the PPAR-γ activation pathway. The obtained results showed that WLIP exerted strong anti-proliferative effect on K562 cells. Moreover, WLIP was found to increase apoptosis and induce G0/Gl arrest. Modeling results from the Surflex-Dock program suggested that PPAR-γ might be the potential anti-tumor target of WLIP, which was confirmed by the experiments that WLIP increased the activity in luciferase reporter assay and the expression of PPAR-γ in Western blot. Besides, WLIP was able to down-regulate the expression of Bcl-xL, Cyclin-D1 in K562 cells. In summary, our novel observations suggested that WLIP might have a potential implication in cancer prevention and treatment, and also showed for the first time that the anti-tumor effect of WLIP might be mediated through modulation of the PPAR-γ activation pathway.
PMID: 24401848 DOI: 10.1016/j.intimp.2013.12.026 Int Immunopharmacol. 2014 Mar;19(1):37-44. doi: 10.1016/j.intimp.2013.12.026. Epub 2014 Jan 5. ncbi.nlm.nih.gov

Cytotoxic constituents of Lasiosphaera fenzlii on different cell lines and the synergistic effects with paclitaxel.
Gao J, Wang LW, Zheng HC, Damirin A, Ma CM.
Abstract
The fruit body of Lasiosphaera fenzlii was found to show cytotoxicity on cancer cells during a preliminary screening. Repeated column chromatography of the fungal methanol extract resulted in the isolation of six compounds identified as 5α,8α-epidioxy-ergosta-6,22-dien-3β-ol (1), 5α,8α-epidioxy-ergosta-6,9(11),22-trien-3β-ol (2), 5α-ergosta-7,22-dien-3β-ol (3), 5α-ergosta-7,22-dien-3-one (4), ergosta-7,22-dien-3β,5α,6β-triol (5) and 6-dihydroxy-2,3-dihydro-1H-isoindol-1-one (6). The two peroxide compounds, 1 and 2, showed cytotoxic activity and compound 1 was selectively cytotoxic to cancer cells. Furthermore, compound 1 synergised the cytotoxicity of paclitaxel on Hela cells by increasing intracellular accumulation of paclitaxel in cancer cells but not in normal cells.
PMID: 26382563 DOI: 10.1080/14786419.2015.1075526  Nat Prod Res. 2016 Aug;30(16):1862-5. doi: 10.1080/14786419.2015.1075526. Epub 2015 Sep 18. ncbi.nlm.nih.gov

Isoindolones from Lasiosphaera fenzliiReich. and Their Bioactivities
Wen-Wen Lü, Yun-Jia Gao, Ming-Zhi Su, Zhou Luo, Wei Zhang, Guo-Bing Shi, Qing-Chun Zhao
Abstract
Two new, 1 and 2, along with one known isoindolone, 3, were isolated from the AcOEt extract of Lasiosphaera fenzlii Reich. The structures of these compounds were determined as 4,6-dihydroxy-1H-isoindole-1,3(2H)-dione (1), 4,6-dihydroxy-2,3-dihydro-1H-isoindol-1-one (2), and clitocybin A (3) on the basis of chemical and spectroscopic evidences. The bioactivity assays revealed that all of them were devoid of significant cytotoxicities against tumor cells, whereas 1 exhibited potent antiangiogenic activity by inhibiting the secretion of vascular endothelial growth factor (VEGF) in A549 cells. First published: 22 January 2013 https://doi.org/10.1002/hlca.201200279 onlinelibrary.wiley.com