Research
Triterpenoids from Pulsatilla chinensis.
Ye WC1, Ji NN, Zhao SX, Liu JH, Ye T, McKervey MA, Stevenson P.
Abstract
A new lupane type triterpenic acid, pulsatillic acid, and two new lupane type
triterpenoid glycosides, pulsatilloside A and B, along with the known 23-hydroxybetulinic
acid were isolated from the roots of Pulsatilla chinensis. Their structures
were characterized as 3-oxo-23-hydroxy-lup-20(29)-en-28-oic acid, 3 beta, 23-dihydroxy-lup-20(29)-en-28-oic
acid 3-O-alpha-L-arabinopyranoside and 3 beta, 23-dihydroxy-lup-20(29)-en-28-oic
acid 28-O-beta-D-glucopyranosyl-(1-->6)-beta-D-glucopyranoside on the basis
of hydrolysis and spectral evidence including two-dimensional relay HOHAHA,
one-dimensional multiple relay COSY and ROESY NMR techniques. Pulsatillic acid
exhibited cytotoxic activities against P-388, Lewis lung carcinoma and human
large-cell lung carcinoma.
PMID: 8768325 Phytochemistry. 1996 Jun;42(3):799-802. ncbi.nlm.nih.gov
Antischistosomal Properties of Hederacolchiside A1 Isolated from Pulsatilla
chinensis
Naixin Kang, Wenhua Shen, Hongwei Gao, Yulin Feng, Weifeng Zhu, Shilin Yang,
Yanli Liu,*, Qiongming Xu,* and Di Yu ID
Abstract:
Background: Schistosomiasis is a major neglected disease for which the current
control strategy involves mass treatment with praziquantel, the only available
drug. Hence, there is an urgent need to develop new antischistosomal compounds.
Methods: The antischistosomal activity of hederacolchiside A1 (HSA) were determined
by total or female worm burden reductions in mice harboring Schistosoma japonicum
or S. mansoni. Pathology parameters were detected on HSA against 1-day-old S.
japonicum-harboring mice. Moreover, we confirmed the antischistosomal effect
of HSA on newly transformed schistosomula (NTS) of S. japonicum in vitro. Results:
HSA, a natural product isolated from Pulsatilla chinensis (Bunge) Regel, was
initially corroborated to possess promising antischistosomal properties. We
demonstrated that HSA had high activity against S. japonicum and S. mansoni
less in 11 days old parasites harbored in mice. The antischistosomal effect
was even more than the currently used drugs, praziquantel, and artesunate. Furthermore,
HSA could ameliorate the pathology parameters in mice harboring 1-day-old juvenile
S. japonicum. We also confirmed that HSA-mediated antischistosomal activity
is partly due to the morphological changes in the tegument system when NTS are
exposed to HSA. Conclusions: HSA may have great potential to be an antischistosomal
agent for further research.
MDPI Molecules Admin/Downloads
New lupane glycosides from Pulsatilla chinensis.
Ye W, Zhang Q, Hsiao WW, Zhao S, Che CT.
Abstract
Two new lupane glycosides along with five known triterpenoids were isolated
from the roots of Pulsatilla chinensis (Ranunculaceae). The structures of the
new glycosides were determined to be 3-O-beta-D-glucopyranosyl(1-->3)-alpha-L-arabinopyranosyl-23-hydroxybetulinic
acid 28-O-alpha-L-rhamnopyranosyl(1-->4)-beta-D-glucopyranosyl(1-->6)-beta-D-glucopyranosyl
ester (pulsatilloside D, 6) and 3-O-[beta-D-glucopyranosyl(1-->4)][alpha-L-rhamnopyranosyl(1-->2)]-alpha-L-arabinopyranosyl-23-hydroxybetulinic
acid 28-O-alpha-L-rhamnopyranosyl(1-->4)-beta-D-glucopyranosyl(1-->6)-beta-D-glucopyranosyl
ester (pulsatilloside E, 7) by spectroscopic analysis and chemical methods.
The compounds were evaluated for cytotoxic activities against K-562 human leukemia
and HeLa cells.
PMID: 11859478 DOI: 10.1055/s-2002-20254
Planta Med. 2002 Feb;68(2):183-6.
ncbi.nlm.nih.gov
Giardia intestinalis: effects of Pulsatilla chinensis extracts on trophozoites.
Li LD, Li WC, Liu CW, Shi WJ, Gong PT, Li JH, Zhang GC, Yang J, Li H, Zhang
XC.
Abstract
Pulsatilla chinensis is a medicinal root plant that has been used to treat a
wide range of disease conditions. Our study determined the antiprotozoal activity
of various P. chinensis extracts and fractions against Giardia intestinalis
including their effects on parasite growth, cell viability, adherence, and morphology.
Ethyl acetate extracts (IC50 = 257.081 μg/ml) were the most active to inhibit
the growth of G. intestinalis followed by aqueous extract (PWE), saponins, and
n-butanol extract. The PWE and ethyl acetate extract inhibited G. intestinalis
trophozoites adherence after 3 h of incubation and killed almost 50 % of the
parasite population in a time-dependent manner. Changes in morphology, presence
of precipitates in the cytoplasm, dissolved cytoplasm with large vacuole, break
of flagella and ventral disk, membrane blebs, and intracellular and nuclear
clearance of the treated trophozoites were observed by scanning and transmission
electron microscopy. We demonstrated that P. chinensis induced these changes
in G. intestinalis morphology and consequently has potential therapeutic use
against giardiasis.
PMID: 22814769 DOI: 10.1007/s00436-012-3035-2
Parasitol Res. 2012 Nov;111(5):1929-35. doi: 10.1007/s00436-012-3035-2. Epub
2012 Jul 20. ncbi.nlm.nih.gov
Effect of Pulsatilla chinensis (Bunge) Regel saponins against juvenile
and adult Schistosoma japonicum in vitro. [Article in Chinese]
Chen YQ, Zhang QY, Li XR, Zhuge HX, Yang SL, Xu QM.
Abstract
OBJECTIVE:
To explore the effect of Pulsatilla chinensis (Bunge) Regel saponins (PRS) against
juvenile and adult Schistosoma japonicum and to compare its efficacy with praziquantel
(PZQ) in vitro.
METHODS:
3 h, 7 d, 14 d schistosomula and 42 d adult schistosomes were incubated with
0, 1, 5, 10, 20 and 30 microg/ml PRS for 4, 24, 48 and 72 hours, then the states
of them were observed. The changes of the surface of S. japonicum incubated
with 30 microg/ml PRS and PZQ within 4 hours were observed by a scanning electron
microscope.
RESULTS:
The sensitivity of 3 h, 7 d, 14 d schistosomula and adults of S. japonicum to
1, 5, 10, 20, 30 microg/ml PRS displayed a time and dose dependence. All the
worms died in 30 microg/ml PRS after 4 hours. The dead worm body appeared a
gray-white color accompanied with their altered morphogenesis and opaque body.
The tegumental surface of adults with different degrees of damages was observed
by the electron microscope within 4 hours affected by PRS in vitro.
CONCLUSIONS:
The effects of PRS against S. japonicum in different developmental stages in
vitro show that PRS may eventually have a therapeutic potential in the treatment
or prevention of S. japonicum infection and is expected to become a new anti-schistosome
drug.
PMID: 24490394 Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi. 2013 Dec;25(6):604-9.
ncbi.nlm.nih.gov
An active molecule from Pulsatilla chinensis, Pulsatilla saponin A,
induces apoptosis and inhibits tumor growth of human colon cancer cells without
or with 5-FU.
Xu L, Cheng G, Lu Y, Wang S.
Abstract
Colon cancer is one of the common types of digestive malignancy. The efficacy
of the first-line chemotherapy drug for colon cancer, fluorouracil (5-FU), remains
limited in clinical settings due to poor efficacy and significant side effects.
In the present study, the anticancer activity of an active compound from Pulsatilla
chinensis extracts, Pulsatilla saponin A (PsA), was isolated and examined in
vitro and in vivo. It was demonstrated that PsA significantly inhibited the
growth of human colon cancer HT-29 cells. This inhibitory activity was also
observed when the compound was tested in a colon cancer xenograft mouse model.
Additionally, the synergic antitumor effects of PsA and 5-FU on colon cancer
cells were observed. Using annexin V and terminal deoxynucleotidyl transferase
2'-deoxyuridine 5'-triphosphate nick end labeling assays, it was demonstrated
that levels of apoptosis induction in HT-29 cells treated with PsA or 5-FU were
significantly increased compared with the untreated control cells (P<0.05).
Western blot analyses were then performed, and the results revealed an increase
in tumor protein 53 and cleaved caspase 9, and a decrease in B-cell lymphoma
2 protein expressions in PsA and PsA + 5-FU treated colon cancer cells compared
with the vehicle-treated (PBS) cells. In summary, PsA exhibited anticancer activity
in human colon cancer cells in vitro and in vivo, in isolation and synergistically
with 5-FU, through apoptosis induction.
PMID: 28529594 PMCID: PMC5431750 DOI: 10.3892/ol.2017.5884
Oncol Lett. 2017 May;13(5):3799-3802. doi: 10.3892/ol.2017.5884. Epub 2017 Mar
21. ncbi.nlm.nih.gov