Bidens tripartita.   Bur marigold, Burr marigold, Water agrimony   Family: Asteraceae    
PART USED: Aerial parts- gathered during the flowering period.
ACTIONS
GROUP:  Herbs for trauma and recovery- Hemostatic
1. Antihemorrhagic.[1]
2. Astringent.[1,2,3]
3. Diaphoretic.[3]
4. Diuretic.
INDICATIONS
1. Bleeding -Menorrhagia. Metrorrhagia. Peptic ulcer. Ulcerative colitis.[1,2,3] Respiratory tract bleeding hemoptysis.
2. Urinary disorders generally including gravel and stones,[1] and hematuria.[1,3]
3. Liver disorders
4. Gout.[3]
SPECIFIC INDICATIONS: Specific hematuria.[1]
COMBINATIONS
- Treatment of the digestive tract, use with Acorus or Ginger.
PREPARATIONS: 3X/day
Dried Herb  2-4 g, [1,2] or by infusion.[1]
Infusion   40-80 ml.
Fluid Extract 1:1 in 25% alcohol  1.5-3 ml.[1,2]
Tincture 1:5 in 45% alcohol  1-2 ml.[1,2]

ORIGIN: Britain and Europe.
HABITAT: Damp places.
DESCRIPTION: Grows up to 60 cm tall, upright, much branched. Leaves; opposite, lanceolate, coarsely serrate, composed of three leaflets. Flowers; small, solitary, heads of composite flowers composed of yellow disc florets.
References
Inner Path can not take any responsibility for any adverse effects from the use of plants. Always seek advice from a professional before using a plant medicinally.
Constituents.
Research.
Used in the USSR for treating alopecia. This may be because of the effects of flavonoids on capillary blood flow.[1]
References
[1] Potter's New Cyclopaedia of Botanical Drugs and Preparations  R.C. Wren Revised by Elizabeth M. Williamson and Fred J Evans. First published in Great Britain in 1988 and reprinted in 1989 and 1994 by the C. W. Daniel Company Limited. 1 Church Path, Saffron Walden Essex. Published 1988 Printed and bound by Biddles, Guildford ISBN 085207 1973.

Experimental researches on acute toxicity of a Bidens tripartita extract in mice - preliminary investigations
R B Sandu 1, L Tartau, A Miron, M Zagnat, C M Ghiciuc, C E Lupusoru
Abstract
Plants take up an important place in traditional medicine and scientific research confirmed properties about their use as alternative therapy. Bidens tripartita, commonly known as Three-lobe Beggarticks, Three-part Beggarticks, Trifid Bur-marigold, is a flowering plant in the genus Bidens, family Compositae, subfamily Asteroideae. Evaluation of the chemical composition of this plant has revealed the presence of flavonoids, xanthophylls, volatile oil, acetylene and polyacetylene, sterols, aurones, chalcones, caffeine and tannins.
Aim: Theoretical data investigation regarding Bidens tripartita plant and experimental researches on acute toxicity of an original extract in mice.
Material and methods: The vegetal product of Bidens tripartita used for study was obtained by maceration and extraction in alcohol, and its chemical composition was determined. Acute toxicity of the alcoholic extract of Bidens tripartita was assessed by median lethal dose (LD50) calculation, using a limit dose test of up- and- down procedure at a limit dose of 2000mg/kbw after intraperitoneal administration in mice.
Results: In the alcoholic extract of Bidens tripartita different active principles were identified: tannins, anthracene derivatives, triterpenes, coumarins, antocyanosides. The toxicity of plant product was evaluated by different characteristic signs for the mouse which can be retained as toxicity elements of the extract. Using the intraperitoneal route, the animals showed dose-dependent signs of toxicity, ranging from lack of appetite, depression, immobility and respiratory distress to death. Single-dose intraperitoneal LD50 value of the alcoholic Bidens tripartita extract in mice was 4038 mg/kg. No macroscopic changes were seen in the organs of mice that died following extract administration. Histopathological lesions were not found in all examined organs.
Conclusions: The obtained LD50 value classifies the study plant extract as slightly toxic according to Hodge and Sterner toxicity scale. We determined the low toxic dose at a rate of 4038 mg of body weight for the alcoholic extract of this medicinal plant. These results suggest that the alcoholic extract of Bidens tripartita is relatively safe toxicologically when administered intraperitoneally, and this product could be used with some degree of safety to continue the investigation for in vivo
Rev Med Chir Soc Med Nat Iasi. Oct-Dec 2012;116(4):1230-4. PMID: 23700917 pubmed.ncbi.nlm.nih.gov

Evaluation of the effects of Bidens tripartita extracts and their main constituents on intestinal motility - An ex vivo study
Marta Mendel, Magdalena Chlopecka, Urszula Latek, Wojciech Karlik, Monika Tomczykowa, Jakub Strawa, Michal Tomczyk
Abstract
Ethnopharmacological relevance: Based on traditional medicine, infusions of Bidens species (Asteraceae) have been successfully used in the treatment of acute and chronic enteritis. Additionally, ethnopharmacological reports demonstrating the gastrointestinal, gastroprotective, anti-inflammatory, antiulcerogenic and immunomodulatory potency of Bidens tripartita Linn. (Asteraceae) and its constituents make the plant a particularly interesting herbal drug candidate for the supportive treatment of functional gastrointestinal and motility disorders.
Aim of the study: The study aimed to verify the effects of B. tripartita and its main flavonoid constituents on intestinal contractility patterns under ex vivo conditions.
Materials and methods: The effects of B. tripartita preparations and their main flavonoids were identified using an alternative model of porcine isolated jejunum specimens. Using LC-ESI-MS, the effects of six different standardized extracts, aqueous (BT1), methanolic 50% (BT2), methanolic (BT3), diethyl ether (BT4), ethyl acetate (BT5) and butanol (BT6) (0.001-0.1 mg/mL), as well as three pure isolated flavonoids, luteolin (LUT), cynaroside (CYN) and flavanomarein (ION) (0.001-100 µM), were evaluated towards spontaneous and acetylcholine-induced motility.
Results and conclusion: s: The results showed the potent prokinetic effects of the B. tripartita extracts and their flavonoids on jejunum smooth muscle. The myocontractile effect was observed on both spontaneous and acetylcholine-induced contractility. There were no substantial differences in the magnitude of myocontractile effects between all six extracts with the exception of the butanol extract which seemed to have a slightly stronger prokinetic effect than the other extracts. The use of extracts at the highest tested concentrations provoked an approximately 1.5-fold increased reaction to acetylcholine compared to the control treatment. The myocontractile effect of the single flavonoids justifies the hypothesis that these secondary metabolites are responsible for the prokinetic activity of all the tested extracts. Among the tested flavonoids, CYN appeared to be the most potent ingredient of B. tripartita; the increase in the response to acetylcholine in the presence of this compound exceeded 250% of the control reaction. In view of the obtained results, the range of functional gastrointestinal disorders in which B. tripartita could be expected to bring benefits include the predominantly constipative phases of irritable bowel syndrome and dyspeptic complaints in which treatment protocols usually involve gastroprokinetics.
Comparative Study J Ethnopharmacol 2020 Sep 15;259:112982. doi: 10.1016/j.jep.2020.112982. Epub 2020 May 19. PMID: 32442590 DOI: 10.1016/j.jep.2020.112982 pubmed.ncbi.nlm.nih.gov